This is certainly especially real for immunohistochemistry in which ten% NBF continues to be proven to be a bad fixative (Fig. two). Some issues with the usage of 10% NBF like a fixative are already corrected by the introduction of other experimental methods for instance antigen retrieval (Shi et al. 2001) and the use of modest amplicons in RT-Q-PC
The incubation time in liquid paraffin should be lengthy more than enough to get ample infiltration, but not any more than needed in order to reduce prolonged exposure on the tissue to warmth. For a similar explanation, it is recommended the temperature of the liquid paraffin ought to be not more than two–four C over the paraffin's melting positi
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